Immunofluorescence on circulating tumor cells (CTCs) is an essential technique in oncology because it allows the multiplexed analysis of several cellular markers simultaneously. This ability to detect several specific biomarkers in a single preparation offers a detailed and comprehensive view of CTCs, thus facilitating the study of their heterogeneity and the evaluation of their role in tumor evolution.
Immunofluorescence MCF-7 K 17 19 CD45 DAPI
Immunofluorescence on CTCs is a crucial tool to explore the molecular characteristics of circulating tumor cells. It provides detailed information on the surface profiles of CTCs, facilitating the identification of specific tumor cells in the blood.
Blood sample
< 4 hours
Cells fixed and analyzed on the isolation support
Cytological
staining
Immunofluorescence
Immunocytochemistry
Fluorescence in situ hybridization
Blood sample
< 3 days
Cytological
staining
Immunofluorescence
Immunocytochemistry
Fluorescence in situ hybridization
Blood sample
< 3 days
ou
Cells fixed detached
from the isolation support for analysis in suspension or on slide
Cytological
staining
Immunofluorescence
Immunocytochemistry
Fluorescence in situ hybridization
Blood sample
< 4 hours
ou
Cells fixed detached
from the isolation support for analysis in suspension or on slide
Cytological
staining
Immunofluorescence
Immunocytochemistry
Fluorescence in situ hybridization
Blood sample
< 4 hours
Living cells detached
from the isolation support for suspension analysis
Cytological
staining
Immunofluorescence
Immunocytochemistry
Fluorescence in situ hybridization
Blood sample < 3 days
or
Fixed cells detached
from the isolation support for analysis in suspension or on slide
Blood sample < 4 hours
or
Fixed cells detached
from the isolation support for analysis in suspension or on slide
ScreenCell kits, compatible with immunofluorescence on CTCs, guarantee high quality results thanks to precise detection of biological markers and a high recovery rate, thanks to our optimized protocols.
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