Immunofluorescence staining on cancer cells is an essential technique in oncology because it allows the multiplexed analysis of several cellular markers simultaneously. This ability to detect several specific biomarkers in a single preparation offers a detailed and comprehensive view of CTCs, thus facilitating the study of their heterogeneity and the evaluation of their role in tumor evolution.
Immunofluorescence Merge 20x
Immunofluorescence staining on CTCs is a crucial tool to explore the molecular characteristics of circulating tumor cells. It provides detailed information on the surface profiles of CTCs, facilitating the identification of specific tumor cells in the blood.
Blood sample
< 4 hours
Cells fixed and analyzed on the isolation support
Cytological
staining
Immunofluorescence
Immunocytochemistry
Fluorescence in situ hybridization
Blood sample
< 3 days
Cytological
staining
Immunofluorescence
Immunocytochemistry
Fluorescence in situ hybridization
Blood sample
< 3 days
ou
Cells fixed detached
from the isolation support for analysis in suspension or on slide
Cytological
staining
Immunofluorescence
Immunocytochemistry
Fluorescence in situ hybridization
Blood sample
< 4 hours
ou
Cells fixed detached
from the isolation support for analysis in suspension or on slide
Cytological
staining
Immunofluorescence
Immunocytochemistry
Fluorescence in situ hybridization
Blood sample
< 4 hours
Living cells detached
from the isolation support for suspension analysis
Cytological
staining
Immunofluorescence
Immunocytochemistry
Fluorescence in situ hybridization
Blood sample < 3 days
or
Fixed cells detached
from the isolation support for analysis in suspension or on slide
Blood sample < 4 hours
or
Fixed cells detached
from the isolation support for analysis in suspension or on slide
ScreenCell® kits compatible with immunofluorescence staining on CTCs deliver high-quality results, combining precise biomarker detection with a high recovery rate, thanks to our optimized protocols.
Ref : EpCAM-expressing circulating tumor cells in colorectal cancer – DOI: 10.5301/ijbm.5000284
Results from immunofluorescence experiments demonstrated that, overall, EpICD and/or EpEX was expressed in 176 CTCs detected by ScreenCell, while the CellSearch® system was able to capture only 10 CTCs.
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